Chan T L, Tsoneva M T
Genetika. 1980;16(8):1495-7.
The changes of mitotic activity and sister chromatid exchanged (SCE) of cultured human lymphocytes under the effect of streptolysin "O" have been studied. Lymphocyte cultures from 9 normal donors were treated with 4 concentrations of streptolysin "O" (0.05, 0.1 0.2 and 0.3 E/ml). Mitotic index in culture (33 100 cells were observed) decreased in inverse proportion to a dose of streptolysin "O" (p = 0.001). Analysis of 455 metaphases (102 control samples and 353 treated with the toxin) showed that streptolysin "O" induced significant increase in the frequency of SCE in cells and in chromosomes (p = 0.001). The frequency of SCE was highest at the lowest dose of toxin (0.05 E/ml) and decreased gradually according to the raising concentrations of toxin in cultures (p = 0.001). The data obtained permit to conclude that streptolysin "O" in concentrations used induces sister chromatid exchanges as well as inhibits mitotic activity in human lymphocytes in vitro.
研究了在链球菌溶血素“O”作用下培养的人淋巴细胞有丝分裂活性和姐妹染色单体交换（SCE）的变化。用4种浓度的链球菌溶血素“O”（0.05、0.1、0.2和0.3 E/ml）处理9名正常供体的淋巴细胞培养物。培养物中的有丝分裂指数（观察33100个细胞）与链球菌溶血素“O”的剂量成反比降低（p = 0.001）。对455个中期相（102个对照样本和353个用毒素处理的样本）的分析表明，链球菌溶血素“O”诱导细胞和染色体中SCE频率显著增加（p = 0.001）。SCE频率在毒素最低剂量（0.05 E/ml）时最高，并随着培养物中毒素浓度的升高而逐渐降低（p = 0.001）。所获得的数据表明，所用浓度的链球菌溶血素“O”在体外可诱导人淋巴细胞发生姐妹染色单体交换并抑制其有丝分裂活性。
