Watson K, Arthur H, Blakey M
J Bacteriol. 1980 Aug;143(2):693-702. doi: 10.1128/jb.143.2.693-702.1980.
Spontaneous and drug-induced respiration-deficient mutants were isolated from the thermophilic enteric yeasts Torulopsis bovina and Saccharomyces telluris. The biochemical properties of these yeasts were compared with those of the two naturally occurring respiration-deficient thermophilic yeasts T. pintolopesii and Candida slooffii. Succinate dehydrogenase was not detected in mitochondrial fractions from C. slooffii, but was present in all other species. Cytochrome c oxidase, succinate oxidase, and reduced nicotinamide adenine dinucleotide oxidase were not detected in C. slooffii, T. pintolopesii, and the respiration-deficient mutants. Low-temperature cytochrome spectra revealed the presence of cytochromes aa3, b, c1, and c in T. bovina and S. telluris; cytochromes b, c1, and c in C. slooffii and T. pintolopesii; and cytochromes c1 and c in the spontaneous respiration-deficient mutants. Palmitoleic and oleic acids were the major fatty acids in all the species. It was noteworthy that T. pintolopesii was rich in lauric and myristic acids. CsCl equilibrium centrifugation experiments showed the presence in all the yeasts of a light-buoyant-density (1.6785 to 1.6837-g/cm3) deoxyribonucleic acid band which was identified as mitochondrial deoxyribonucleic acid by its selective elimination on treatment of cells with ethidium bromide. The latter result indicated that the spontaneous respiration-deficient mutants were similar to cytoplasmic petite mutants of S. cerevisiae. Although classical assimilation and fermentation tests indicated that the spontaneous respiration-deficient mutants were strains of T. pintolopesii, it was concluded, on the basis of marked physiological and biochemical differences, that this was not the case.
从嗜热肠道酵母牛球拟酵母（Torulopsis bovina）和土生酿酒酵母（Saccharomyces telluris）中分离出自发性和药物诱导的呼吸缺陷型突变体。将这些酵母的生化特性与两种天然存在的呼吸缺陷型嗜热酵母平托洛普球拟酵母（T. pintolopesii）和斯洛菲念珠菌（Candida slooffii）的生化特性进行了比较。在斯洛菲念珠菌的线粒体组分中未检测到琥珀酸脱氢酶，但在所有其他物种中均有存在。在斯洛菲念珠菌、平托洛普球拟酵母和呼吸缺陷型突变体中未检测到细胞色素c氧化酶、琥珀酸氧化酶和还原型烟酰胺腺嘌呤二核苷酸氧化酶。低温细胞色素光谱显示，牛球拟酵母和土生酿酒酵母中存在细胞色素aa3、b、c1和c；斯洛菲念珠菌和平托洛普球拟酵母中存在细胞色素b、c1和c；自发呼吸缺陷型突变体中存在细胞色素c1和c。棕榈油酸和油酸是所有物种中的主要脂肪酸。值得注意的是，平托洛普球拟酵母富含月桂酸和肉豆蔻酸。氯化铯平衡离心实验表明，所有酵母中均存在一条轻浮力密度（1.6785至1.6837 g/cm³）的脱氧核糖核酸带，通过用溴化乙锭处理细胞后其选择性消除，该带被鉴定为线粒体脱氧核糖核酸。后一结果表明，自发呼吸缺陷型突变体与酿酒酵母的细胞质小菌落突变体相似。尽管经典的同化和发酵试验表明自发呼吸缺陷型突变体是平托洛普球拟酵母的菌株，但基于明显的生理和生化差异得出结论，情况并非如此。
