Semler P, Rippl H J
Infusionsther Klin Ernahr. 1981 Apr;8(2):82-7.
Blood samples of four volunteers were prepared for amino acid analysis in different ways using 39 methods. The result was that after initial deproteinisation of the blood samples different storage temperatures ranging from -18 degrees C to -78 degrees C and different sorting periods from 2 to 6 months did no influence the amino acid spectra. Storage at -4 degrees C did not show a clear result, however slightly decreased glutamine levels. It is possible to allow blood samples to stand at room temperature for further processing for at least 60 minutes, without adverse influence on the analytical result.
If it is impossible to carry out a quantitative amino acid analysis immediately after blood withdrawal, the blood sample can be stored for several months at not more than -18 degrees C provided that within about 90 minutes after blood withdrawal centrifugation and deproteinisation has been carried out.
使用39种方法以不同方式制备了4名志愿者的血样用于氨基酸分析。结果是，血样经过初始脱蛋白处理后，在-18℃至-78℃的不同储存温度以及2至6个月的不同分选时间段内，均未对氨基酸谱产生影响。在-4℃储存未显示出明确结果，但谷氨酰胺水平略有下降。血样可以在室温下放置至少60分钟以便进一步处理，而不会对分析结果产生不利影响。
如果在采血后无法立即进行定量氨基酸分析，只要在采血后约90分钟内进行离心和脱蛋白处理，血样可以在不超过-18℃的温度下储存数月。
