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血浆氨基酸分析:延迟样本制备及储存的影响

Plasma amino-acids analysis: effects of delayed samples preparation and of storage.

作者信息

Schaefer A, Piquard F, Haberey P

出版信息

Clin Chim Acta. 1987 Apr 30;164(2):163-9. doi: 10.1016/0009-8981(87)90067-2.

DOI:10.1016/0009-8981(87)90067-2
PMID:3594909
Abstract

This paper describes changes occurring in plasma amino-acid concentrations of: samples which are not prepared for analysis immediately after blood collection and samples stored for 5 to 6 months in the form of either plasma, or of deproteinized plasma, or of deproteinized and at pH 2.2 buffered plasma. Results showed that in order to avoid these changes, plasma should be deproteinized and buffered as soon as possible after blood collection. When analysis could not be performed immediately, storage of samples in a freezer at -18 degrees C in the form of deproteinized and at pH 2.2 buffered plasma showed after 5 to 6 months the best recovery of the initial concentrations, but did not exclude all changes.

摘要

本文描述了以下几种样本血浆氨基酸浓度的变化情况

采血后未立即准备用于分析的样本,以及以血浆、脱蛋白血浆或pH值为2.2的脱蛋白缓冲血浆形式储存5至6个月的样本。结果表明,为避免这些变化,采血后应尽快对血浆进行脱蛋白和缓冲处理。若无法立即进行分析,将样本以pH值为2.2的脱蛋白缓冲血浆形式于-18℃冷冻保存5至6个月后,初始浓度的回收率最佳,但仍无法排除所有变化。

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