A simple and rapid method in the determination of urinary estrogen to creatinine ratio during pregnancy.

The fluorometry reported in this paper is a method which fulfils the necessary precision, accuracy, sensitivity and specificity for the determination of the urinary estrogen to creatinine ratio of pregnant women. With this method: (1) whole procedures are completed by single capillary specimen; (2) consequently a very small amount of urine is required; (3) only 35 min are spent completing the in vitro measurement, and (4) urinary estrogen of 5 weeks' gestation can be estimated due to its high sensitivity and specificity. Normal cases and abnormal pregnancies are discussed utilizing this method.