日本血吸虫：人血清中抗虫卵抗体放射免疫测定法的应用

Schistosoma japonicum: use of a radioimmunoassay for anti egg antibodies in human sera.

作者信息

Tapales F P, Mitchell G F, Garcia E G, Cruise K M, Valdez C A, Anders R F

出版信息

Southeast Asian J Trop Med Public Health. 1981 Mar;12(1):19-23.

PMID:7196090

Abstract

A solid-phase radioimmunoassay (RIA) was developed for schistosomiasis japonica using extracted egg antigens and compared with circumoval precipitin test (COPT) results on 20 sera from known S. japonicum-infected individuals and on 10 control sera. The quantitative RIA very clearly differentiated between infected and uninfected individuals with highest titers being obtained in teenagers. However, in the series employed, information relevant to immunodiagnosis of S. japonicum infection was contained in the non-quantitative but simple COPT and little was apparently added to the quantitative but expensive RIA.

摘要

利用提取的虫卵抗原开发了一种用于日本血吸虫病的固相放射免疫测定法（RIA），并将其与环卵沉淀试验（COPT）的结果进行比较，这些结果来自20份已知感染日本血吸虫个体的血清以及10份对照血清。定量RIA能够非常清晰地区分感染个体和未感染个体，青少年的滴度最高。然而，在所采用的系列研究中，与日本血吸虫感染免疫诊断相关的信息包含在非定量但简单的COPT中，而定量但昂贵的RIA显然没有增加太多信息。