Garcia E G, Tapales F P, Valdez C A, Mitchell G F, Tiu W U
Southeast Asian J Trop Med Public Health. 1981 Sep;12(3):384-95.
The circumoval precipition test (COPT) is a simple and inexpensive immunodiagnostic test for schistosomiasis japonica which, in the Philippines, has high sensitivity and specificity. Lack of standardization does, however, increase the variability of the test. Parameters which influence the COPT have been examined using large numbers of sera from known S. japonicum infected individuals. In this series of experiments, optimal conditions were determined to be as follows using 2 drops of neat serum and incubation at 37 degrees C in a sealed slide chamber; - approximately 100 eggs from 55 or 60 days infected rabbits for a 24 to 48 hour incubation period. COP reactions (i.e. precipitates associated with eggs) were much less obvious when either immature eggs or eggs obtained from long-term infected rabbits were used. The results emphasize the prime importance of the source of Schistosoma japonicum eggs in the performance of a standardized COPT.
环卵沉淀试验（COPT）是一种用于日本血吸虫病的简单且廉价的免疫诊断试验，在菲律宾，该试验具有较高的敏感性和特异性。然而，缺乏标准化确实会增加试验的变异性。已使用大量来自已知感染日本血吸虫个体的血清对影响COPT的参数进行了研究。在这一系列实验中，确定的最佳条件如下：使用2滴纯血清，在密封载玻片盒中于37℃孵育； - 来自感染55或60天兔子的约100个卵，孵育24至48小时。当使用未成熟卵或从长期感染兔子获得的卵时，COP反应（即与卵相关的沉淀物）明显较少。结果强调了日本血吸虫卵来源在标准化COPT性能中的首要重要性。
