Tarin D, Price J E
Cancer Res. 1981 Sep;41(9 Pt 1):3604-9.
This communication reports experiments demonstrating that some sites in which tumor cells lodge reproducibly fail to support secondary colony formation by a particular tumor, even though cells from the same tumor are already proven to have high colonization potential in other organs. The effect is not an expression of nonspecific hostility to tumor growth, since cells from certain other tumors readily colonize the same site. Cell suspensions obtained by disaggregation of a series of naturally occurring murine mammary tumors were each inoculated by four different routes into separate batches of syngeneic animals, and the resulting degree and distribution of colonization were studied 90 days later at autopsy. Standard doses of 1 million viable tumor cells were injected either i.p. or s.c. into the tail vein or the hepatic portal vein. It was found that some tumors could reproducibly colonize by all routes, whereas others could colonize only by a few, and the combination of sites colonized varied from tumor to tumor; still others were unable to grow in any site. Cells from nonneoplastic lactating mammary glands did not establish any colonies. We have demonstrated previously that individual naturally occurring mammary tumors differ in their pulmonary colonization potentials after i.v. inoculation and that the potential of a given tumor is an intrinsic property of its constituent cells. The current findings are evidence that the microenvironment of an organ can inhibit or permit expression of this intrinsic potential and that the degree and sites of colonization are thus the results of interaction between tumor and organ-specific factors. It was also found that circulatory anatomy partially influenced the distribution of colonies and that colonization of distant organs after blood-borne dissemination is distinct from general tumor transplantability.
本通讯报道了一些实验，这些实验表明，某些肿瘤细胞可重复性地定植的部位，却无法支持特定肿瘤形成继发性集落，尽管同一肿瘤的细胞已被证明在其他器官中具有很高的定植潜力。这种效应并非对肿瘤生长的非特异性敌意的表现，因为来自某些其他肿瘤的细胞很容易在同一部位定植。通过解离一系列自然发生的小鼠乳腺肿瘤获得的细胞悬液，分别通过四种不同途径接种到同基因动物的不同批次中，并在90天后尸检时研究由此产生的定植程度和分布。将100万个活肿瘤细胞的标准剂量通过腹腔内、皮下、尾静脉或肝门静脉注射。结果发现，一些肿瘤可以通过所有途径可重复性地定植，而另一些肿瘤只能通过少数途径定植，并且定植部位的组合因肿瘤而异；还有一些肿瘤在任何部位都无法生长。来自非肿瘤性泌乳乳腺的细胞没有形成任何集落。我们之前已经证明，个体自然发生的乳腺肿瘤在静脉注射后的肺定植潜力不同，并且给定肿瘤的潜力是其组成细胞的固有特性。目前的研究结果证明，器官的微环境可以抑制或允许这种固有潜力的表达，因此定植的程度和部位是肿瘤与器官特异性因素相互作用的结果。还发现循环解剖结构部分影响集落的分布，并且血行播散后远处器官的定植与一般肿瘤移植性不同。
