Lawless D, Brown D H, Hübner K F, Colyer S P, Carlton J E, Hayes R L
Cancer Res. 1978 Dec;38(12):4440-4.
A Glycoprotein, particularly high in tumors, has been extracted from Morris 5123C rat hepatomas and purified. The compound constitutes a major binding component for 67Ga in this hepatoma. It has a molecular weight of approximately 45,000. Its molecular weight was determined by sodium dodecyl sulfate:polyacrylamide gel electrophoresis and by Sephadex G-200 superfine gel filtration. The steps involved in its extraction and purification include ultrafiltration, gel filtration through Sephadex G-200 superfine, ion-exchange chromatography on diethylaminoethyl Sephadex A-50, and hydroxylapatite chromatography. The homogeneity of the compound was established by gel electrophoresis. The NH2-terminal residue, the percentage of nitrogen, the nonamino carbohydrate content, and the amino acid composition are reported.
从莫里斯5123C大鼠肝癌中提取并纯化出一种在肿瘤中含量特别高的糖蛋白。该化合物是这种肝癌中67Ga的主要结合成分。其分子量约为45000。它的分子量通过十二烷基硫酸钠：聚丙烯酰胺凝胶电泳和葡聚糖G - 200超细凝胶过滤法测定。其提取和纯化步骤包括超滤、通过葡聚糖G - 200超细进行凝胶过滤、在二乙氨基乙基葡聚糖A - 50上进行离子交换色谱以及羟基磷灰石色谱。通过凝胶电泳确定了该化合物的纯度。报告了其氨基末端残基、氮的百分比、非氨基碳水化合物含量和氨基酸组成。
