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[天蓝色链霉菌A3(2)放线菌噬菌体介导的质粒SCP2转移]

[Plasmid SCP2 transfer by Streptomyces coelicolor A3(2) actinophages].

作者信息

Mkrtumian N M, Lomovskaia N D

出版信息

Genetika. 1981;17(8):1398-405.

PMID:7196857
Abstract

The ability of VP5 and phi c31 actinophages of Streptomyces coelicolor A3(2) to transfer plasmid SCP2 genes was tested. Infection with both phages led to formation of variants having the traits characteristics of plasmid bearing strains. The fertility properties of the variants suggested the presence of SCP2 in the autonomous state. Analysis of extrachromosomal DNA isolated from these variants demonstrated its identity with the plasmid DNA from the donor strain. Based on characteristics of the variants obtained, it was suggested that the whole SCP2 plasmid had been transferred by actinophages via general transduction.

摘要

测试了天蓝色链霉菌A3(2)的VP5和φc31肌动噬菌体转移质粒SCP2基因的能力。用这两种噬菌体感染均导致形成具有携带质粒菌株特征性状的变体。这些变体的育性特性表明SCP2以自主状态存在。对从这些变体中分离的染色体外DNA的分析表明,其与供体菌株的质粒DNA相同。根据所获得变体的特征,推测整个SCP2质粒已被肌动噬菌体通过普遍性转导进行了转移。

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Genetika. 1981;17(8):1398-405.
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