Pitzel L, Bischoff C, König A
Exp Brain Res. 1981;44(2):229-31. doi: 10.1007/BF00237344.
The in vitro release of oxytocin (OXT) from posterior pituitary lobes (PPL) of adult female rats is linked to the stage of the animals' sexual cycles. After incubation in normal Locke's solution (K+ 5,6 mM), the basal OXT release from the PPL of rats on diestrus 1 and estrus amounted to 2.25 +/- 0.53 ng/lobe/10 min (mean +/- S.E.M.) and 4.71 +/- 0.61 ng/lobe/10 min, respectively. Excess K+ (56 mM/l) in the presence of Ca++ (2.2 mM/l) increased OXT liberation from the PPl of diestrous and estrous rats to 12.41 +/- 2.65 ng/lobe/10 min und 36.33 +/- 6.18 ng/lobe/10 min, respectively. When Ca++ was omitted from the incubation medium, the K+-excess induced OXT release from the PPL of estrous rats decreased to 21.54 +/- 2.65 ng/lobe/10 min whereas no change occurred in the OXT-release from the rats' PPL on diestrus 1 in the presence of Ca++. The results indicate a cycle-dependent release and suggest that OXT plays a role in the reproductive processes.
成年雌性大鼠垂体后叶（PPL）中催产素（OXT）的体外释放与动物的性周期阶段有关。在正常洛克氏溶液（K⁺ 5.6 mM）中孵育后，处于动情间期1和发情期的大鼠PPL的基础OXT释放量分别为2.25±0.53 ng/叶/10分钟（平均值±标准误）和4.71±0.61 ng/叶/10分钟。在存在Ca²⁺（2.2 mM/L）的情况下，过量的K⁺（56 mM/L）使动情期和发情期大鼠PPL的OXT释放量分别增加到12.41±2.65 ng/叶/10分钟和36.33±6.18 ng/叶/10分钟。当孵育培养基中省略Ca²⁺时，过量K⁺诱导的发情期大鼠PPL的OXT释放量降至21.54±2.65 ng/叶/10分钟，而在存在Ca²⁺的情况下，处于动情间期1的大鼠PPL的OXT释放量没有变化。结果表明存在周期依赖性释放，并提示OXT在生殖过程中起作用。
