Fumero S, Berruto G P, Pelizzola D, Grilli S, Buttazzi C, Di Fronzo G, Ronchi E, Bozzetti C, Mori P, Concolino G, Marocchi A, Robustelli Della Cuna G, Zibera C, Cerrutti G, Ros A, Piffanelli A
Tumori. 1981 Aug;67(4):301-6. doi: 10.1177/030089168106700406.
This article presents results of the first Italian quality control program for determining the estradiol receptor on lypophilized guinea pig and calf uteri. Despite considerable variability in quantitative terms, the results concur in ability to define samples as positive or negative for receptor content. One of the parameters that most strongly influences accuracy of determination of receptor concentration is protein assay. The evaluation of several lyophilized preparations at scalar concentrations permitted identification, by linear regression, for each laboratory of the systematic and non-systematic variables. More comparable results will be forthcoming when a standardized methodology program has been fully adopted.
本文介绍了首个意大利质量控制项目的结果，该项目旨在测定冻干豚鼠和小牛子宫中的雌二醇受体。尽管在定量方面存在相当大的变异性，但结果在将样本定义为受体含量阳性或阴性的能力上是一致的。对受体浓度测定准确性影响最大的参数之一是蛋白质测定。对标量浓度的几种冻干制剂进行评估，通过线性回归，可为每个实验室识别系统变量和非系统变量。当完全采用标准化方法程序时，将获得更具可比性的结果。
