苯丙酮酸三氯化铁试验的改进
Improvement of the FeCl3 test for phenylpyruvic acid.
作者信息
Cassidei L, Dell'atti A, Sciacovelli O
出版信息
Clin Chim Acta. 1978 Dec 1;90(2):121-7. doi: 10.1016/0009-8981(78)90513-2.
A simple alternative colorimetric method has been developed for quantitative determination of phenylpyruvic acid in urine based on the improved stability of the ferric complex of enol-phenylpyruvate in water-dimethylsulfoxide 50% vol. mixed solvent. The method can be applied directly to urine or to a dry ether-extract of urine. Operating on the dry ether-extract of urine, detection of phenylpyruvic acid is possible at an amount of as low as 1 mg/100 ml. Whereas, if urine samples are processed, detection is possible for a concentration of as low as 5 mg/100 ml and quantitative determination for a concentration greater than 10 mg/100 ml. The test carried out on a diaper wet with urine retains the same sensitivity and shows instantaneous development of the green colour.
基于烯醇 - 苯丙酮酸铁配合物在50%（体积）水 - 二甲基亚砜混合溶剂中的稳定性提高，已开发出一种简单的比色法用于尿液中苯丙酮酸的定量测定。该方法可直接应用于尿液或尿液的干乙醚提取物。对尿液的干乙醚提取物进行操作时，低至1 mg/100 ml的苯丙酮酸即可被检测到。而如果对尿液样本进行处理，低至5 mg/100 ml的浓度即可被检测到，浓度大于10 mg/100 ml时可进行定量测定。在尿湿的尿布上进行测试，其灵敏度相同，并呈现出绿色的即时显色。
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