Bo W J, Krueger W A, Rudeen P K, Symmes S K
Anat Rec. 1982 Feb;202(2):255-60. doi: 10.1002/ar.1092020210.
The purpose of this study was to compare the effects of different dosages of ethanol on ovarian morphology and function. Holtzman rats, 20 days old, were divided into groups as follows: The rats in Group I were autopsied at 20 days of age, and those in Group II were placed on ad libitum chow and water diet; the rats in Groups III and V were fed on a liquid diet containing 2.5% or 5% ethanol respectively; Groups IV and VI were pair-fed controls to Groups III and V, respectively. Rats in Groups II, III, IV, and VI were maintained on the diets for 50-55 days and killed at late proestrus-estrus, while the animals in Group V did not exhibit estrous cycles and were killed on day 55 of treatment. The average increase in body weights of rats in Groups II, III, and IV was significantly greater than the increase in body weights of rats given 5% ethanol or their pair-fed controls. In the rats treated with 5% ethanol, vaginal opening was significantly delayed from the controls, estrous cycles were absent, ovarian weights were similar to those of the 20-day-old rats, ovaries contained corpora lutea of only one estrus, uteri weighted less than controls, and histologically, the uteri and vaginae were similar to those of 20-day-old rats. However, in the rats treated with 2.5% ethanol, all of the parameters were similar to those of the controls. The average serum alcohol level for the rats on the 5% ethanol diet was 249 mg%; the serum alcohol levels were at the lower limit of detection for the rats on the 2.5% ethanol diet. The data show that ovarian function was suppressed in the rats that received the 5% ethanol but not in rats on the 2.5% ethanol diet.
本研究的目的是比较不同剂量乙醇对卵巢形态和功能的影响。将20日龄的霍尔茨曼大鼠分为以下几组：第一组大鼠在20日龄时进行解剖，第二组大鼠自由进食和饮水；第三组和第五组大鼠分别喂食含2.5%或5%乙醇的流质饮食；第四组和第六组分别为第三组和第五组的配对喂食对照组。第二组、第三组、第四组和第六组的大鼠维持饮食50 - 55天，并在动情后期 - 发情期处死，而第五组的动物未出现发情周期，在治疗第55天处死。第二组、第三组和第四组大鼠的平均体重增加显著大于给予5%乙醇的大鼠或其配对喂食对照组的体重增加。在接受5%乙醇治疗的大鼠中，阴道开口明显比对照组延迟，无发情周期，卵巢重量与20日龄大鼠相似，卵巢仅含一个发情期的黄体，子宫重量小于对照组，组织学上，子宫和阴道与20日龄大鼠相似。然而，在接受2.5%乙醇治疗的大鼠中，所有参数均与对照组相似。5%乙醇饮食组大鼠的平均血清酒精水平为249mg%；2.5%乙醇饮食组大鼠的血清酒精水平处于检测下限。数据表明，接受5%乙醇的大鼠卵巢功能受到抑制，而接受2.5%乙醇饮食的大鼠则未受影响。
