Gozzo S, Oliverio A, Salvati S, Serlupi-Crescenzi G, Tagliamonte B, Tomassi G
Food Chem Toxicol. 1982 Apr;20(2):153-7. doi: 10.1016/s0278-6915(82)80240-8.
Five groups of female mice were fed from day 14 of pregnancy and throughout lactation on synthetic diets differing only in the lipid component, which constituted 10% of the diet and was derived from (a) the lipid fraction extracted from yeast grown on n-alkanes, (b) commercial soya lecithin, (c) fatty acids isolated from yeast lipids (off-chain fatty acids), (d) lipids extracted from yeast grown on glucose or (e) margarine (8%), corn oil (1%) and the unsaponifiable fraction (1%) from yeast grown on n-alkanes. A control diet (9% margarine and 1% corn oil) was fed to a sixth group of pregnant mice and to the offspring of all the groups from weaning to 60 days after birth. A battery of behavioural tests was performed on the offspring of the six groups between days 1 and 16 after birth (reflex activities), between days 2 and 21 (locomotor activity) and at 60 days of age (avoidance learning). An acceleration of postnatal behavioural maturation was evident in the pups of animals fed lipids from yeast grown on n-alkanes and of those fed soya lecithin, indicating the involvement of phospholipids and odd-chain fatty acids in the aspects of development studied.
将五组雌性小鼠从怀孕第14天开始直至整个哺乳期，喂食仅在脂质成分上有所不同的合成饲料，脂质占饲料的10%，分别来源于：(a) 从以正构烷烃为碳源生长的酵母中提取的脂质部分；(b) 市售大豆卵磷脂；(c) 从酵母脂质中分离出的脂肪酸（支链脂肪酸）；(d) 从以葡萄糖为碳源生长的酵母中提取的脂质；或 (e) 人造黄油（8%）、玉米油（1%）以及从以正构烷烃为碳源生长的酵母中提取的不皂化物部分（1%）。给第六组怀孕小鼠以及所有组的后代从断奶至出生后60天喂食对照饲料（9%人造黄油和1%玉米油）。在出生后第1天至16天（反射活动）、第2天至21天（运动活动）以及60日龄（回避学习）对六组小鼠的后代进行了一系列行为测试。喂食以正构烷烃为碳源生长的酵母脂质的母鼠所产幼崽以及喂食大豆卵磷脂的母鼠所产幼崽，出生后行为成熟加速，这表明磷脂和奇数链脂肪酸在所研究的发育方面发挥了作用。
