Betz J M, Blogoslawski W J
J Pharm Sci. 1982 Apr;71(4):463-5. doi: 10.1002/jps.2600710425.
Brine shrimp (Artemia salina L.) were exposed to concentrations of live Gonyaulax tamarensis var. excavata cells ranging from 0 to 13,400 cells/ml in unialgal culture. The shrimp ingested the dinoflagellate cells and an LD50 determination was made. The shrimp had a 72-hr LD50 of 1258 G. tamarensis cells/ml, which was calculated to correspond to 73,385 micrograms/kg of body weight. This figure can be compared with an oral LD50 value of 2100 micrograms/kg for the mouse. Since it requires one-tenth as much toxin to kill a mouse (LD50 approximately 0.04 mg) as to kill an Artemia (LD50 approximately 0.4 mg), the whole cell G. tamarensis bioassay is a poor alternative to the current mouse assay.
卤虫（Artemia salina L.）在单种培养中暴露于浓度范围为0至13400个细胞/毫升的活塔玛亚历山大藻（Gonyaulax tamarensis var. excavata）细胞中。卤虫摄取了这些甲藻细胞，并进行了半数致死剂量（LD50）测定。卤虫的72小时LD50为1258个塔玛亚历山大藻细胞/毫升，经计算相当于73385微克/千克体重。这个数字可以与小鼠口服LD50值2100微克/千克进行比较。由于杀死一只小鼠（LD50约为0.04毫克）所需的毒素量是杀死一只卤虫（LD50约为0.4毫克）所需毒素量的十分之一，因此塔玛亚历山大藻全细胞生物测定法并非当前小鼠测定法的理想替代方法。
