Sharp C S, Bessman A N, Wagner F W, Garland D
Diabetes Care. 1978 Sep-Oct;1(5):289-92. doi: 10.2337/diacare.1.5.289.
Information on the incidence and nature of the causative organisms in the infected tissues of patients with diabetic gangrene is scanty. Studies in which material for culture was obtained from the presenting lesion reveal multiple organisms in host isolates. No data are available regarding the bacterial flora of the deep infected tissue itself, uncontaminated by surface organisms. In this investigation 58 specimens from 52 patients were obtained utilizing aerobic and anaerobic culture techniques. In the surgical theater, material was obtained from the infected deep tissues using careful aseptic dissection techniques. All patients had been on antibiotic therapy from 2 to 10 days before the study. An average of 2.3 organisms per specimen was found. The predominant organisms were Proteus sp., Enterococcus, Staphylococcus aureus, and other enterobacteriacae in that order. Anaerobes were isolated in 27 per cent of cases, but never as the only organism. Prior antibiotic therapy did not eradicate infection in infected diabetic gangrene.
关于糖尿病坏疽患者感染组织中致病微生物的发病率和性质的信息很少。从出现的病变中获取培养材料的研究表明，宿主分离物中有多种微生物。目前尚无关于未被表面微生物污染的深部感染组织本身的细菌菌群的数据。在这项研究中，利用需氧和厌氧培养技术从52例患者身上获取了58份标本。在手术室中，采用仔细的无菌解剖技术从感染的深部组织中获取材料。所有患者在研究前已接受2至10天的抗生素治疗。每个标本平均发现2.3种微生物。主要微生物依次为变形杆菌属、肠球菌、金黄色葡萄球菌和其他肠杆菌科细菌。27%的病例中分离出厌氧菌，但从未作为唯一的微生物。先前的抗生素治疗未能根除糖尿病坏疽感染。
