Smiseth O A, Refsum H, Vik-Mo H, Mjøs O D
Clin Physiol. 1982 Feb;2(1):39-50. doi: 10.1111/j.1475-097x.1982.tb00005.x.
The mechanism for reduced myocardial ischaemic injury by hyaluronidase was studied in open chest anaesthetized dogs. Repeated coronary artery occlusions were performed and the effect of hyaluronidase (225 NF units per kg) was studied during infusion of noradrenaline 0.125 mg/kg . min. Ischaemic injury was measured as the sum of ST-segment elevations (sigma ST) at 10-15 sites. Regional myocardial blood flow was determined by tracer microspheres. Blood for metabolic studies was sampled from a local coronary vein draining ischaemic tissue and from the coronary sinus draining predominantly non-ischaemic tissue. Hyaluronidase reduced sigma ST and increased subepicardial and transmural blood flow in ischaemic myocardium, but flow was not significantly changed in the ischaemic subendocardium or in non-ischaemic myocardium. Hyaluronidase had no significant effect on arterio-local venous differences of oxygen, glucose, lactate or free fatty acids across the ischaemic myocardium. In conclusion, reduction of myocardial ischaemic injury by hyaluronidase can be explained by increased collateral blood flow and not by an effect on fluxes of substrates across the ischaemic myocardium.
在开胸麻醉犬中研究了透明质酸酶减轻心肌缺血性损伤的机制。进行反复冠状动脉闭塞，并在输注去甲肾上腺素0.125mg/kg·min期间研究透明质酸酶（225 NF单位/kg）的作用。缺血性损伤以10 - 15个部位的ST段抬高总和（σST）来衡量。通过示踪微球测定局部心肌血流量。用于代谢研究的血液从引流缺血组织的局部冠状静脉以及主要引流非缺血组织的冠状窦采集。透明质酸酶降低了σST，并增加了缺血心肌的心外膜下和透壁血流量，但缺血心内膜下或非缺血心肌的血流量没有显著变化。透明质酸酶对缺血心肌的氧、葡萄糖、乳酸或游离脂肪酸的动-局部静脉差值没有显著影响。总之，透明质酸酶减轻心肌缺血性损伤可通过增加侧支血流量来解释，而不是通过对缺血心肌底物通量的影响。
