Hamburger J, Lustigman S, Siongok T K, Ouma J H, Mahmoud A A
J Immunol. 1982 Oct;129(4):1711-4.
Preliminary analysis and purification of glycoproteins from Schistosoma haematobium eggs were carried out with a small quantity of antigenic material obtained from the urine of infected human patients. A soluble egg extract was 125I-labeled and was fractionated by lectin affinity chromatography for separating egg glycoproteins. The crude glycoprotein fraction was run on SDS-PAGE to yield three polydisperse peaks with Rf values of 0.31, 0.57, and 0.84. 125I-labeled egg glycoproteins were further fractionated by ion exchange chromatography to yield four peaks or shoulders. One of these peaks constituted the major labeled egg glycoprotein of S. haematobium (MEGL-H) in a relatively pure form as determined by SDS-PAGE, and its estimated m.w. was 70,000. This glycoprotein was consistently and highly reactive serologically with a serum pool from schistosomiasis haematobia patients by a Farr-type radioimmunoassay (RIA). A limited cross-specificity study of inhibition RIA indicated that S. mansoni eggs contain components that cross-react only partially with MEGL-H. These results focus attention on MEGL-H as a potential serodiagnostic probe.
利用从感染患者尿液中获取的少量抗原物质，对埃及血吸虫卵中的糖蛋白进行了初步分析和纯化。将可溶性虫卵提取物进行¹²⁵I标记，然后通过凝集素亲和层析对虫卵糖蛋白进行分离。粗糖蛋白组分在SDS-PAGE上进行电泳，得到三个多分散峰，Rf值分别为0.31、0.57和0.84。¹²⁵I标记的虫卵糖蛋白进一步通过离子交换层析进行分离，得到四个峰或肩峰。其中一个峰以相对纯的形式构成了埃及血吸虫主要的标记虫卵糖蛋白（MEGL-H），通过SDS-PAGE测定其估计分子量为70,000。通过Farr型放射免疫分析（RIA），这种糖蛋白与埃及血吸虫病患者的血清池在血清学上始终具有高度反应性。抑制RIA的有限交叉特异性研究表明，曼氏血吸虫卵含有仅与MEGL-H部分交叉反应的成分。这些结果使人们将注意力集中在MEGL-H作为一种潜在的血清学诊断探针上。
