Kummerfeld H L, Vosburgh J K, Lorton S P, Foote R H
Fertil Steril. 1981 Feb;35(2):218-21.
In vitro sperm migration assays were performed using bovine spermatozoa and cervical mucus. Experiments were designed to test the effects of storage temperature, method of storage, duration of storage, and source of cervical mucus. Significant variation in migration of spermatozoa was due both to differences in mucus samples and to short-term mucus storage at temperatures ranging from ambient to -196 degrees C. The parallel-orienting effect of cervical mucus on migrating sperm was shown to be a major factor in quantitative assays based upon migration distance. Thus, comparisons of migration among different specimens of semen likely will be biased unless the tests are run simultaneously. Implications of these results are discussed relative to the performance of quantitative sperm migration assays in the clinical or research laboratory.
使用牛精子和宫颈黏液进行了体外精子迁移试验。实验旨在测试储存温度、储存方法、储存持续时间以及宫颈黏液来源的影响。精子迁移的显著差异既归因于黏液样本的差异，也归因于在从环境温度到 -196 摄氏度范围内的短期黏液储存。宫颈黏液对迁移精子的平行定向作用在基于迁移距离的定量试验中被证明是一个主要因素。因此，除非同时进行测试，否则不同精液样本之间迁移情况的比较可能会有偏差。相对于临床或研究实验室中定量精子迁移试验的表现，讨论了这些结果的意义。
