Lorton S P, Kummerfeld H L, Foote R H
Fertil Steril. 1981 Feb;35(2):222-5.
A synthetic migration medium for capillary sperm migration studies was developed. Parallel sperm migration in 1.8% polyacrylamide cross-linked with 0.042% N,N'-methylene bis acrylamide was similar to sperm migration in bovine cervical mucus. Bull spermatozoa varying widely in migration distances in bovine cervical mucus maintained similar relative migration distances in this synthetic medium. The advantages of the synthetic medium are its availability in large quantities, its uniformity, and its stability. There was no change in parallel sperm migration distances in the synthetic medium stored at 4 degrees C for up to 4 months. Use of this synthetic medium for human or bovine sperm migration studies would appear to overcome problems associated with the variability of cervical mucus.
开发了一种用于毛细管精子迁移研究的合成迁移介质。在与0.042% N,N'-亚甲基双丙烯酰胺交联的1.8%聚丙烯酰胺中平行精子迁移与在牛宫颈粘液中的精子迁移相似。在牛宫颈粘液中迁移距离差异很大的公牛精子在这种合成介质中保持相似的相对迁移距离。合成介质的优点是大量可得、均匀且稳定。在4℃下储存长达4个月的合成介质中，平行精子迁移距离没有变化。将这种合成介质用于人类或牛精子迁移研究似乎可以克服与宫颈粘液变异性相关的问题。
