Refsum N, Lowery C, Nordøy A
Haemostasis. 1981;10(1):3-13. doi: 10.1159/000214382.
Unsaturated fatty acids (FA) were bound to human albumin in a molar ratio of 2 and were then added to platelet-rich plasma (PRP), increasing the total FA concentration by 1 mM. Separate FA and FA paired with di-homo-gamma-linolenic acid (DHLA) and arachidonic acid (AA), respectively, were incubated with PRP for 5 and 90 min, and platelet aggregation and platelet factor 3 (PF3) activity were measured. DHLA inhibited adenosine-diphosphate- and collagen-induced platelet aggregation after 5 min, an effect which was enhanced after 90 min. The effects of AA, which stimulated platelet aggregation and increased PF3 activity, were partially counteracted by DHLA after 90 min. When linoleic acid and AA were incubated with PRP in equimolar concentrations for 90 min, an inhibition of collagen-induced platelet aggregation was observed which was not observed after incubation with these FA separately. This study indicates that exogenous albumin-bound AA and DHLA may be used directly by the platelet cyclo-oxygenase pathway before significant incorporation into the platelet phospholipids has occurred.
不饱和脂肪酸（FA）以2的摩尔比与人类白蛋白结合，然后添加到富血小板血浆（PRP）中，使总FA浓度增加1 mM。分别将FA以及分别与二高-γ-亚麻酸（DHLA）和花生四烯酸（AA）配对的FA与PRP孵育5分钟和90分钟，并测量血小板聚集和血小板因子3（PF3）活性。DHLA在5分钟后抑制二磷酸腺苷和胶原诱导的血小板聚集，90分钟后这种作用增强。90分钟后，刺激血小板聚集并增加PF3活性的AA的作用被DHLA部分抵消。当亚油酸和AA以等摩尔浓度与PRP孵育90分钟时，观察到对胶原诱导的血小板聚集有抑制作用，而分别与这些FA孵育后未观察到这种抑制作用。这项研究表明，外源性白蛋白结合的AA和DHLA在大量掺入血小板磷脂之前可能直接被血小板环氧化酶途径利用。
