Nordøy A, Svensson B, Hoak J C
Eur J Clin Invest. 1979 Feb;9(1):5-10. doi: 10.1111/j.1365-2362.1979.tb01661.x.
This study was carried out to evaluate the effects of albumin-bound fatty acids on the anti-platelet effects of endothelial cells. Primary cultures of human endothelial cells (ECM), grown in confluent monolayers, were incubated with plasma or growth medium enriched with albumin-bound fatty acids (FA) for 2-20 h. The effects of ECM on ADP-induced platelet aggregation (PA) and collagen-induced PA and prostaglandin synthesis in platelet-rich plasma were tested. ECM released an inhibitor of platelet reactions which resembled the activity of PGI2 (prostacyclin). The inhibitory activity was increased by preincubation of ECM with arachidonic acid (AA). A moderate decrease of the activity was obtained by incubation with long-chain saturated, monoenoic and dienoic unsaturated fatty acids. A pronounced decrease of the inhibitor was obtained by incubation with di-homo-gamma-linolenic acid (DHLA). Paired combinations of AA with the other fatty acids in the incubation medium partially restored the inhibitory activity obtained by the separate FA. The stimulation of the inhibitor by AA was dose dependent and high concentrations of AA reduced this activity. The present study indicates that the quantity and quality of the plasma free fatty acids can affect the endothelial cells' ability to act as a non-thrombogenic surface.
本研究旨在评估白蛋白结合脂肪酸对内皮细胞抗血小板作用的影响。将生长成汇合单层的人内皮细胞（ECM）原代培养物与富含白蛋白结合脂肪酸（FA）的血浆或生长培养基孵育2 - 20小时。测试了ECM对富含血小板血浆中ADP诱导的血小板聚集（PA）、胶原诱导的PA以及前列腺素合成的影响。ECM释放出一种血小板反应抑制剂，其活性类似于前列环素（PGI2）。用花生四烯酸（AA）预孵育ECM可增强其抑制活性。与长链饱和脂肪酸、单不饱和脂肪酸和二不饱和脂肪酸孵育会使活性适度降低。与二高 - γ - 亚麻酸（DHLA）孵育会使抑制剂显著减少。在孵育培养基中，AA与其他脂肪酸的配对组合部分恢复了单独使用FA时获得的抑制活性。AA对抑制剂的刺激呈剂量依赖性，高浓度的AA会降低这种活性。本研究表明，血浆游离脂肪酸的数量和质量会影响内皮细胞作为非血栓形成表面的能力。
