体外纯脑微管蛋白形成微管。葡聚糖和聚乙二醇的影响。
Microtubule formation by pure brain tubulin in vitro. The influence of dextran and poly(ethylene glycol).
作者信息
Herzog W, Weber K
出版信息
Eur J Biochem. 1978 Nov 2;91(1):249-54. doi: 10.1111/j.1432-1033.1978.tb20959.x.
Abstract
Homogeneous brain tubulin, free of microtubule associated proteins, forms microtubules in polymerization buffers containing 7.5--15% dextran T10 or 2--4.5% poly(ethylene glycol) type 6000. In both systems the resulting microtubules are sensitive to colchicine and low temperature. The critical tubulin concentration in both polymerization systems can be as low as 0.25 mg/ml.
摘要
不含微管相关蛋白的均一脑微管蛋白，在含有7.5% - 15%葡聚糖T10或2% - 4.5%聚乙二醇6000的聚合缓冲液中形成微管。在这两种体系中，所形成的微管对秋水仙碱和低温敏感。两种聚合体系中的临界微管蛋白浓度可低至0.25毫克/毫升。
Zotero 插件