Moss C W
J Chromatogr. 1981 Jan 9;203:337-47. doi: 10.1016/s0021-9673(00)80305-2.
The use of gas-liquid chromatography (GLC) in clinical and diagnostic bacteriology laboratories has increased significantly in recent years. GLC analysis of metabolic products from bacterial growth and chemical components of bacterial cells has provided useful information for rapid detection and identification of several bacterial groups or species. The use of short-chain acid products and cellular fatty acid composition for identifying and classifying Pseudomonas species and other medically important gram-negative non-fermentative bacteria is illustrated. Application of the flexible, fused, silica-glass, capillary column for increased resolution of bacterial fatty acids is also discussed.
近年来，气液色谱法（GLC）在临床和诊断细菌学实验室中的应用显著增加。对细菌生长代谢产物和细菌细胞化学成分进行的气液色谱分析，为快速检测和鉴定多个细菌类群或菌种提供了有用信息。文中举例说明了利用短链酸产物和细胞脂肪酸组成来鉴定和分类假单胞菌属菌种及其他具有医学重要性的革兰氏阴性非发酵菌。还讨论了使用柔性熔融石英玻璃毛细管柱提高细菌脂肪酸分离度的应用。
