Barksdale L, Linder R, Sulea I T, Pollice M
J Clin Microbiol. 1981 Feb;13(2):335-43. doi: 10.1128/jcm.13.2.335-343.1981.
A search has been made for corynebacterial phospholipase D, "ovis toxin," a sphingomyelinase (phosphatidylcholine phosphohydrolase, EC 3.1.4.4), among a wide variety of corynebacteria. Phospholipase D activity has been found in strains exhibiting the biochemical properties characteristic of Corynebacterium pseudotuberculosis or of Corynebacterium ulcerans and in no other species of Corynebacterium. Methods for the assay of phospholipase D as a sphingomyelinase and methods for screening for phospholipase D in the presence of Corynebacterium equi on washed sheep blood agar are discussed.
已在多种棒状杆菌中寻找棒状杆菌磷脂酶D(“羊毒素”,一种鞘磷脂酶(磷脂酰胆碱磷酸水解酶,EC 3.1.4.4))。在表现出假结核棒状杆菌或溃疡棒状杆菌特征性生化特性的菌株中发现了磷脂酶D活性,而在其他棒状杆菌物种中未发现。讨论了将磷脂酶D作为鞘磷脂酶进行测定的方法以及在马棒状杆菌存在的情况下在洗涤过的羊血琼脂上筛选磷脂酶D的方法。