检测引起咽外感染的白喉棒状杆菌和溃疡棒状杆菌的白喉毒素核苷酸及氨基酸序列差异。
Detection of differences in the nucleotide and amino acid sequences of diphtheria toxin from Corynebacterium diphtheriae and Corynebacterium ulcerans causing extrapharyngeal infections.
作者信息
Sing Andreas, Hogardt Michael, Bierschenk Suse, Heesemann Jürgen
机构信息
National Consiliary Laboratory on Diphtheria, 80336 Munich, Germany.
出版信息
J Clin Microbiol. 2003 Oct;41(10):4848-51. doi: 10.1128/JCM.41.10.4848-4851.2003.
Abstract
While Corynebacterium ulcerans can mimic classical diphtheria, extrapharyngeal infections are extremely rare. Sequencing of the diphtheria toxin (DT)-encoding tox gene of two C. ulcerans isolates from extrapharyngeal infections revealed differences from C. diphtheriae DT sequences, mainly in the translocation and receptor-binding domains. C. ulcerans supernatants were much less potent than supernatant from C. diphtheriae. A C. ulcerans DT-specific PCR is described below.
摘要
虽然溃疡棒状杆菌可模仿典型的白喉，但咽外感染极为罕见。对两株来自咽外感染的溃疡棒状杆菌分离株的白喉毒素（DT）编码tox基因进行测序，发现其与白喉棒状杆菌DT序列存在差异，主要在易位和受体结合结构域。溃疡棒状杆菌的上清液效力远低于白喉棒状杆菌的上清液。下文介绍一种溃疡棒状杆菌DT特异性PCR。
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