Hommez J, Devriese L A, Vaneechoutte M, Riegel P, Butaye P, Haesebrouck F
Regional Veterinary Laboratory, Torhout, France.
J Clin Microbiol. 1999 Apr;37(4):954-7. doi: 10.1128/JCM.37.4.954-957.1999.
Nonlipophilic corynebacteria associated with clinical and subclinical mastitis in dairy cows were found to belong to four species: Corynebacterium amycolatum, Corynebacterium ulcerans, Corynebacterium pseudotuberculosis, and Corynebacterium minutissimum. These species may easily be confused. However, clear-cut differences between C. ulcerans and C. pseudotuberculosis were found in their acid production from maltotriose and ethylene glycol, susceptibility to vibriostatic agent O129, and alkaline phosphatase. Absence of growth at 20 degrees C and lack of alpha-glucosidase and 4MU-alpha-D-glycoside hydrolysis activity differentiated C. amycolatum from C. pseudotuberculosis and C. ulcerans. The mastitis C. pseudotuberculosis strains differed from the biovar equi and ovis reference strains and from caprine field strains in their colony morphologies and in their reduced inhibitory activity on staphylococcal beta-hemolysin. C. amycolatum was the most frequently isolated nonlipophilic corynebacterium.
解脂棒状杆菌、溃疡棒状杆菌、假结核棒状杆菌和极小棒状杆菌。这些物种可能很容易混淆。然而，在麦芽三糖和乙二醇产酸、对制菌剂O129的敏感性以及碱性磷酸酶方面，发现溃疡棒状杆菌和假结核棒状杆菌之间存在明显差异。在20℃不生长以及缺乏α-葡萄糖苷酶和4MU-α-D-糖苷水解活性可将解脂棒状杆菌与假结核棒状杆菌和溃疡棒状杆菌区分开来。乳腺炎假结核棒状杆菌菌株在菌落形态以及对葡萄球菌β-溶血素抑制活性降低方面，与马鼻疽生物变种和绵羊生物变种参考菌株以及山羊野外菌株不同。解脂棒状杆菌是最常分离出的非亲脂性棒状杆菌。
