Hudson E M, Ramsey R B, Evatt B L
J Lab Clin Med. 1981 Apr;97(4):577-82.
Human platelets labeled with [111In]oxine have been suggested for use in platelet kinetic studies, thrombus localization, detection of atherosclerotic lesions, and other scintigraphic techniques. To demonstrate the subcellular localization of indium-111 in labeled platelets, we did subcellular fractionation and measured the amount of indium-111 localized in major platelet components. Human platelets were labeled with [111In]oxine and disrupted by nitrogen cavitation. The cellular material was fractionated on sucrose density gradients. When the subcellular fractions were analyzed, more than 70% of the indium-111 was located in the cytoplasmic pool of the platelets. Chromatography on Sephadex G-100 of aliquots of the labeled cytosol material showed that most of the indium-111 was associated with components of approximately 46,000 daltons. This demonstrated that indium-111 in human platelets labeled with [111In]oxine is located predominantly in platelet cytosol and is associated with cytoplasmic components.
用[111In]奥克辛标记的人血小板已被建议用于血小板动力学研究、血栓定位、动脉粥样硬化病变检测及其他闪烁显像技术。为了证明111铟在标记血小板中的亚细胞定位，我们进行了亚细胞分级分离，并测量了111铟在主要血小板成分中的含量。用人血小板与[111In]奥克辛进行标记，然后通过氮空化作用使其破裂。细胞物质在蔗糖密度梯度上进行分级分离。当对亚细胞级分进行分析时，超过70%的111铟位于血小板的细胞质池中。对标记的细胞溶质物质的等分试样在Sephadex G - 100上进行色谱分析表明，大部分111铟与大约46,000道尔顿的成分相关。这表明用[111In]奥克辛标记的人血小板中的111铟主要位于血小板细胞质中，并与细胞质成分相关。
