Baker J R, Butler K D, Eakins M N, Pay G F, White A M
Blood. 1982 Feb;59(2):351-9.
In order to examine the subcellular distribution of 111In in 111In-oxine-labeled human and rabbit platelets, we employed a hypothetical grain technique of EM autoradiography analysis. The results indicate that in the rabbit 111In was concentrated within the platelet dense bodies, particularly when the platelets had been labeled in a plasma-free system. Under comparable conditions of labeling, human platelets appeared to accumulate almost all the radiolabel within the cytosol. Using inhibitors of 5-hydroxytrptamine (5-HT) uptake, i.e., cloimipramine, ouabain, sodium fluoride, p-chloromercuribenzoate, and reserpine, we were unable to demonstrate an active uptake process in either species. Both collagen and thrombin were able to cause dose-dependent release of radioactivity from the labeled rabbit platelets only. In the case of collagen, this mimicked endogenous 5-HT release and was inhibited by indomethacin. These results and their implications are discussed.
为了研究铟-111在铟-奥克辛标记的人及兔血小板中的亚细胞分布，我们采用了电子显微镜放射自显影分析的假设颗粒技术。结果表明，在兔体内，铟-111集中在血小板致密体中，特别是当血小板在无血浆系统中标记时。在相同的标记条件下，人血小板似乎将几乎所有的放射性标记物积累在细胞质中。使用5-羟色胺（5-HT）摄取抑制剂，即氯米帕明、哇巴因、氟化钠、对氯汞苯甲酸和利血平，我们无法在任何一种物种中证明有活跃的摄取过程。胶原蛋白和凝血酶都只能引起标记兔血小板中放射性的剂量依赖性释放。就胶原蛋白而言，这模拟了内源性5-HT的释放，并被吲哚美辛抑制。讨论了这些结果及其意义。
