铟 - 111作为一种新型高光子产率γ发射“生理性”血小板标记物的评估。

Evaluation of indium-111 as a new high photon yield gamma-emitting "physiological" platelet label.

作者信息

Scheffel U, McIntyre P A, Evatt B, Dvornicky J A, Natarajan T K, Bolling D R, Murphy E A

出版信息

Johns Hopkins Med J. 1977 Jun;140(6):285-93.

Abstract

In order to evaluate indium-111 (111In) as a "physiological" platelet label, rabbit platelets were labeled in vitro with 111In-8-hydroxyquinoline. The in vivo behavior of 111In labeled platelets was then compared with that of chromium-51 51Cr) labeled platelets in the rabbit. Several important features were evident. First, unlike 51Cr, reasonable platelet labeling efficiency could be achieved with 111In, even when the platelets were suspended in small amounts of autologous plasma. Second, the initial recovery of platelets at 20 minutes after infusion was significantly higher for the 111In labeled platelets than for 51Cr labeled platelets, providing the platelets were suspended and "washed" in plasma. Third, the mean survival time of 111In labeled platelets, as estimated using four different mathematical models, was comparable with that of 51Cr platelets. Finally, the high photon yield of gamma emitting 111In, unlike 51Cr, permits quantitative gamma camera imaging of the in vivo distribution of labeled platelets. Thus, this nuclide possesses advantages as a platelet label that will foster statistically more accurate kinetic research as well as quantitative in vivo distribution studies in humans.

摘要

为了评估铟 - 111（¹¹¹In）作为一种“生理性”血小板标记物，用¹¹¹In - 8 - 羟基喹啉在体外标记兔血小板。然后将¹¹¹In标记血小板在兔体内的行为与铬 - 51（⁵¹Cr）标记血小板的行为进行比较。几个重要特征很明显。首先，与⁵¹Cr不同，即使血小板悬浮在少量自体血浆中，¹¹¹In也能实现合理的血小板标记效率。其次，如果血小板在血浆中悬浮并“洗涤”，输注后20分钟时¹¹¹In标记血小板的初始回收率显著高于⁵¹Cr标记血小板。第三，使用四种不同数学模型估计，¹¹¹In标记血小板的平均存活时间与⁵¹Cr血小板相当。最后，与⁵¹Cr不同，发射γ射线的¹¹¹In的高光子产率允许对标记血小板的体内分布进行定量γ相机成像。因此，这种核素作为血小板标记物具有优势，将有助于在人体中进行统计学上更准确的动力学研究以及定量体内分布研究。