Francesconi M, Meryn S, Moser K, Bauer K
J Clin Chem Clin Biochem. 1981 Jan;19(1):17-20. doi: 10.1515/cclm.1981.19.1.17.
The activity of ribonuclease (E 3.1.4.22) was estimated in sera of 401 normal subjects (normal renal function, no signs of catabolism), age ranging from 16 to 100 years (mean: 50.25 +/- 19.98) with Poly (C) as substrate. Further activity was measured with the substrates Poly (U), Poly (A) and Poly (G) in 10 sera from each age decade between 21-30 years and 81-90 years. Greatest activity was found with Poly (C) as substrate (mean: 15.88 kU/l +/- 9.57). Covariant analysis (age as covariant) revealed no sex-related activity distribution at any age, whereas the age-dependent activity increase was highly significant (p less than 0.001; 11-20 years: 9.48 kU/l +/- 3.07; 81-90 years: 34.72 kU/l +/- 5.88). Activity with the other substrates was very low and constant throughout all ages. It seems to be mandatory to consider the age of the patient when interpreting ribonuclease activity.
以聚（C）为底物，对401名正常受试者（肾功能正常，无分解代谢迹象）的血清中的核糖核酸酶（E 3.1.4.22）活性进行了评估。这些受试者年龄在16至100岁之间（平均：50.25±19.98）。在21至30岁和81至90岁之间的每个年龄十年中，从10份血清中用聚（U）、聚（A）和聚（G）作为底物进一步测量了活性。发现以聚（C）为底物时活性最高（平均：15.88 kU/l±9.57）。协方差分析（年龄作为协变量）显示，在任何年龄都没有与性别相关的活性分布，而年龄依赖性的活性增加非常显著（p＜0.001；11至20岁：9.48 kU/l±3.07；81至90岁：34.72 kU/l±5.88）。在所有年龄段中，其他底物的活性都非常低且恒定。在解释核糖核酸酶活性时，似乎必须考虑患者的年龄。
