Karch F E, Chmielewski K F
J Pharm Sci. 1981 Feb;70(2):229-30. doi: 10.1002/jps.2600700231.
A simple, rapid, and highly sensitive method for the GLC analysis of plasma lidocaine is described. Mepivacaine hydrochloride is added as the internal standard; the plasma is deproteinated and centrifuged, and the supernate is alkalinized and extracted into a small volume of carbon disulfide. The column is connected to a flame-ionization detector, and the column oven temperature is programmed from 130 to 260 degrees. Plasma lidocaine concentrations between 0.04 and 8.0 micrograms/ml can be measured accurately, and there is no interference from the monoethylglycinexylidide or glycinexylidide metabolites of lidocaine or from many commonly used drugs and diagnostic agents. The sensitivity, simplicity, and speed of this assay are important in pharmacokinetic studies of lidocaine.
本文描述了一种用于血浆利多卡因气相色谱分析的简单、快速且高度灵敏的方法。加入盐酸甲哌卡因作为内标；血浆进行去蛋白处理并离心，上清液碱化后用少量二硫化碳萃取。色谱柱连接火焰离子化检测器，柱温箱温度从130℃程序升温至260℃。可准确测定血浆中浓度在0.04至8.0微克/毫升之间的利多卡因，利多卡因的单乙基甘氨酰二甲苯酰胺或甘氨酰二甲苯酰胺代谢物以及许多常用药物和诊断剂均无干扰。该测定方法的灵敏度、简便性和速度在利多卡因的药代动力学研究中很重要。
