Hucker H B, Stauffer S C
J Pharm Sci. 1976 Jun;65(6):926-7. doi: 10.1002/jps.2600650635.
A simple, rapid GLC method for the determination of therapeutic levels of lidocaine in plasma or blood is described. After extraction with benzene from alkalinized plasma, the compound is analyzed by GLC using a nitrogen-sensitive detector. Quantitation is accomplished using an internal standard and a standard curve, which was linear over the 0.1-10-mug range. The limit of detection was approximately 10 ng/ml. Recovery of the parent compound was essentially quantitative. Plasma data are presented to demonstrate the utility of the method.
本文描述了一种简单、快速的气相色谱法，用于测定血浆或血液中利多卡因的治疗浓度。用苯从碱化血浆中萃取后，使用氮敏感检测器通过气相色谱法分析该化合物。使用内标和标准曲线进行定量，该标准曲线在0.1-10微克范围内呈线性。检测限约为10纳克/毫升。母体化合物的回收率基本为定量。给出了血浆数据以证明该方法的实用性。
