Kline B J, Martin M F
J Pharm Sci. 1978 Jun;67(6):887-8. doi: 10.1002/jps.2600670650.
A simple, rapid GLC method for the determination of lidocaine in plasma is described. After addition of mepivacaine as an internal standard, the plasma sample is deproteinated and centrifuged. The supernate is alkalinized, and the lidocaine and internal standard are extracted into a microvolume of carbon disulfide. By using GLC with a flame-ionization detector, linear concentration-response curves were obtained in the 1-6 microgram/ml range. The method can easily determine plasma concentrations at the 250-ng/ml level when a 1-ml plasma sample is analyzed.
本文描述了一种用于测定血浆中利多卡因的简单、快速的气相色谱法。加入甲哌卡因作为内标后，对血浆样品进行去蛋白处理并离心。将上清液碱化，然后将利多卡因和内标物萃取到微量二硫化碳中。使用带有火焰离子化检测器的气相色谱法，在1 - 6微克/毫升范围内获得了线性浓度-响应曲线。当分析1毫升血浆样品时，该方法能够轻松测定250纳克/毫升水平的血浆浓度。
