Reader T A
J Neural Transm. 1981;50(1):13-27. doi: 10.1007/BF01254910.
The rat cerebral cortex was dissected in five regions and analyzed for the catecholamines noradrenaline, adrenaline and dopamine, and for the indoleamine serotonin using sensitive radioenzymatic assay methods with thin-layer chromatography. The noradrenaline concentration was highest in the ventral cortex, lateral to the hypothalamus, had intermediate values for the prefrontal, frontal and parietal cortical areas and was lowest in the occipital cortex. Dopamine levels were also highest in the cortex lateral to the hypothalamus, and moderate in the prefrontal and frontal cortical areas, with the lowest values measured for the occipital cortex. The ratios dopamine/noradrenaline further supports the hypothesis that they are independent transmitters. Traces of adrenaline were measured in all regions examined. The serotonin distribution was found to be non-homogeneous, with the highest values for the prefrontal cortex and ventral cortex lateral to the hypothalamus. The functional significance of these amines and their ratios are discussed in relation to their role as putative modulators of cortical neuronal excitability.
将大鼠大脑皮层分为五个区域进行解剖，采用灵敏的放射性酶测定法结合薄层色谱法，分析其中的儿茶酚胺类物质去甲肾上腺素、肾上腺素和多巴胺，以及吲哚胺类物质5-羟色胺。去甲肾上腺素浓度在下丘脑外侧的腹侧皮层中最高，在额叶前区、额叶和顶叶皮层区域为中等水平，在枕叶皮层中最低。多巴胺水平同样在下丘脑外侧的皮层中最高，在额叶前区和额叶皮层区域为中等水平，在枕叶皮层中测得的值最低。多巴胺/去甲肾上腺素的比值进一步支持了它们是独立递质的假说。在所检查的所有区域中均检测到痕量肾上腺素。发现5-羟色胺的分布不均匀，在额叶前区皮层和下丘脑外侧的腹侧皮层中含量最高。结合这些胺类物质及其比值作为假定的皮层神经元兴奋性调节剂的作用，讨论了它们的功能意义。
