Whatley B J, Green J B, Green M H
J Nutr. 1981 Mar;111(3):432-41. doi: 10.1093/jn/111.3.432.
Sixteen female rabbits were fed one of four diets during lactation: 1) a commercial stock diet; 2) the stock diet with 5% added lard; 3) the stock diet with 5% lard and 0.25% cholesterol; or 4) the stock diet with 15% lard and 1% cholesterol. By days 30-35 of lactation, maternal plasma cholesterol concentrations were increased approximately 10-fold in group 3 and 100-fold in group 4 does compared to does in group 1. Milk cholesterol concentration was similar over lactation for does in groups 1, 2 and 3, but was approximately 2 times higher in group 4 does. Milk triglyceride and protein concentrations and milk intake by the pups were not significantly influenced by maternal diet. Average cholesterol intake of pups nursed by group 4 does was significantly higher than that of other pups. Although plasma cholesterol concentration was significantly increased in group 2, 3 and 4 pups at weaning (age 5 weeks), there was no significant effect of maternal diet on plasma cholesterol at 6, 7 or 11 weeks of age after pups had been weaned to the stock diet. Similarly, liver cholesterol concentration was increased in pups from group 4 does at weaning, but these differences were no longer apparent at 11 weeks of age. These data suggest that severe maternal hypercholesterolemia induced by dietary fat and cholesterol in the rabbit can result in increased milk cholesterol concentration and consequent cholesterol intake by the pups. However, the associated elevations in pup plasma and liver cholesterol levels at weaning did not persist when pups were weaned to a low fat/low cholesterol diet.
16只雌性兔子在哺乳期被喂食四种日粮中的一种：1）市售常规日粮；2）添加5%猪油的常规日粮；3）添加5%猪油和0.25%胆固醇的常规日粮；或4）添加15%猪油和1%胆固醇的常规日粮。在哺乳期第30 - 35天，与第1组母兔相比，第3组母兔的母体血浆胆固醇浓度增加了约10倍，第4组母兔增加了100倍。第1、2和3组母兔在整个哺乳期的乳胆固醇浓度相似，但第4组母兔的乳胆固醇浓度约高2倍。母兔日粮对乳甘油三酯和蛋白质浓度以及幼崽的乳摄入量没有显著影响。由第4组母兔哺育的幼崽的平均胆固醇摄入量显著高于其他幼崽。尽管在断奶时（5周龄）第2、3和4组幼崽的血浆胆固醇浓度显著升高，但在幼崽断奶后改喂常规日粮至6、7或11周龄时，母兔日粮对血浆胆固醇没有显著影响。同样，第4组母兔的幼崽在断奶时肝脏胆固醇浓度升高，但在11周龄时这些差异不再明显。这些数据表明，兔子日粮中的脂肪和胆固醇诱导的严重母体高胆固醇血症可导致乳胆固醇浓度升高，进而导致幼崽胆固醇摄入量增加。然而，当幼崽断奶改喂低脂/低胆固醇日粮时，断奶时幼崽血浆和肝脏胆固醇水平的相关升高并未持续。
