Lin I M, Lei K Y
J Nutr. 1981 Mar;111(3):450-7. doi: 10.1093/jn/111.3.450.
Ninety weanling male Sprague-Dawley rats were randomly allotted into two treatment groups (copper-deficient and adequate; less than 2 mg and 8 mg Cu/kg of diet. Feed and distilled water were provided ad libitum for 16 weeks. After 5 weeks of treatment, the rats were injected intraperitoneally with 6.35 microCI [4-14C]cholesterol/kg body weight. A disappearance curve of the serum cholesterol specific activity (SA) was obtained for each treatment group by killing one animal at 1, 2, 7, 14, 28, 42, 56, 70 and 77 days after the tracer injection. Disappearance curves of serum cholesterol SA were constructed for the rats fed the copper-adequate and copper-deficient diets. Each disappearance curve of cholesterol SA in the serum was subjected to a kinetic two-pool analysis. The size of the fast turning over cholesterol pool (pool A) and the half-life of pool A were significantly greater in rats fed the copper-deficient diet than the controls. In addition, copper deficiency decreased the rate of removal of cholesterol from pool A (KAA), decreased the irreversible removal of cholesterol from pool A (KA) and reduced the rate of transport of cholesterol (KAB) from pool A to the slowly exchangeable pool (pool B). However, copper deficiency did not alter the production rate of cholesterol (PRA) in pool A. Thus, the reduction in the rate of removal of cholesterol from pool A might have been responsible for the hypercholesterolemia observed previously in rats fed copper-deficient diets.
将90只断乳雄性斯普拉格-道利大鼠随机分为两个处理组（缺铜组和正常铜组；日粮中铜含量分别低于2毫克/千克和8毫克/千克）。随意提供饲料和蒸馏水，持续16周。处理5周后，给大鼠腹腔注射6.35微居里[4-¹⁴C]胆固醇/千克体重。通过在注射示踪剂后的第1、2、7、14、28、42、56、70和77天处死一只动物，获得每个处理组血清胆固醇比活性（SA）的消失曲线。为喂食正常铜和缺铜日粮的大鼠构建血清胆固醇SA的消失曲线。对血清中胆固醇SA的每条消失曲线进行动力学双池分析。喂食缺铜日粮的大鼠中，快速周转胆固醇池（池A）的大小和池A的半衰期显著大于对照组。此外，缺铜降低了胆固醇从池A的清除率（KAA），降低了胆固醇从池A的不可逆清除率（KA），并降低了胆固醇从池A向缓慢交换池（池B）的转运率（KAB）。然而，缺铜并未改变池A中胆固醇的生成率（PRA）。因此，池A中胆固醇清除率的降低可能是先前在喂食缺铜日粮的大鼠中观察到的高胆固醇血症的原因。
