Strauss R G
J Clin Lab Immunol. 1980 Mar;3(2):133-5.
Infants and patients with neutropenia provide few neutrophils for study. Accordingly, assays measuring oxidative metabolism (chemiluminescence, glucose-1-C14 oxidation, nitroblue tetrazolium dye reduction and superoxide anion generation) were studied to determine the range of neutrophil concentrations over which results were directly related to the number of neutrophils per reaction, and to define the limits (particularly, the lower one) of cell numbers studied that would yield accurate and comparative data. The chemiluminescence assay was then performed employing low neutrophil concentrations to document that the modified technique could detect a metabolic defect in neutrophils from infants when compared to cells obtained from their mothers and controls.
婴儿和中性粒细胞减少的患者可供研究的中性粒细胞很少。因此，对测量氧化代谢的检测方法（化学发光、葡萄糖-1-C14氧化、硝基蓝四氮唑染料还原和超氧阴离子生成）进行了研究，以确定中性粒细胞浓度范围，在此范围内结果与每个反应中的中性粒细胞数量直接相关，并确定所研究的细胞数量的限度（特别是下限），这将产生准确和可比较的数据。然后采用低中性粒细胞浓度进行化学发光检测，以证明与从母亲和对照者获得的细胞相比，改良技术能够检测出婴儿中性粒细胞中的代谢缺陷。
