Strauss R G
J Clin Pathol. 1981 Jul;34(7):800-2. doi: 10.1136/jcp.34.7.800.
The thiobarbituric acid (TBA) assay for measuring malonaldehyde (MDA) has been applied to many tissues as a simple means of detecting oxidative damage and prostaglandin synthesis. Human neutrophils, cells known to produce prostaglandins and toxic metabolites of oxygen, were studied to determine if this assay could provide a suitable, rapid, screening test to recognise states of metabolic activation, to monitor prostaglandin synthesis, or to identify the presence of oxidative damage. Malonaldehyde could not be detected after a variety of manipulations. Thus, it seems unlikely that the thiobarbituric acid assay, as performed here, will be useful for evaluating oxidative damage in human neutrophils.
用于测定丙二醛（MDA）的硫代巴比妥酸（TBA）测定法已作为检测氧化损伤和前列腺素合成的一种简单方法应用于许多组织。人类中性粒细胞是已知能产生前列腺素和氧毒性代谢产物的细胞，对其进行了研究，以确定该测定法是否能提供一种合适、快速的筛选试验，用于识别代谢激活状态、监测前列腺素合成或鉴定氧化损伤的存在。经过各种处理后均未检测到丙二醛。因此，此处所进行的硫代巴比妥酸测定法似乎不太可能用于评估人类中性粒细胞中的氧化损伤。
