Sleyster E C, Knook D L
Mech Ageing Dev. 1980 Nov-Dec;14(3-4):443-52. doi: 10.1016/0047-6374(80)90015-9.
Purified suspensions of highly viable parenchymal, endothelial and Kupffer cells were prepared from the livers of young (3 months of age) and old (33-34 months of age) female rats. In the cell suspensions, the activities of the multiple forms of acid phosphatase were determined with the substrates 4-methylumbelliferyl phosphate, 1-naphthyl phosphate and p-nitrophenyl phosphate. Most specific enzyme activities increased during aging, but the preference to hydrolyse any of the three substrates did not change. The small age-related changes in effects of the inhibitors fluoride and alloxan were not uniform for the the three liver cell types. The pattern for multiple forms of acid phosphatase obtained after isoelectric focusing did not change with age for Kupffer cells, while one of the two major forms of the enzyme almost disappeared in endothelial cells from old rats. With age, a very pronounced heterogeneous pattern was observed for the enzyme forms in the long-lived parenchymal cells. The increase in heterogeneity in multiple forms of acid phosphatase in aging parenchymal cells may be the result of post-translational modifications.
从年轻（3个月大）和年老（33 - 34个月大）雌性大鼠的肝脏中制备了高活性实质细胞、内皮细胞和库普弗细胞的纯化悬浮液。在细胞悬浮液中，用4 - 甲基伞形酮磷酸酯、1 - 萘基磷酸酯和对硝基苯磷酸酯作为底物测定了多种形式酸性磷酸酶的活性。大多数特异性酶活性在衰老过程中增加，但水解这三种底物中任何一种的偏好没有改变。抑制剂氟化物和四氧嘧啶对三种肝细胞类型的影响与年龄相关的微小变化并不一致。等电聚焦后获得的多种形式酸性磷酸酶的模式，库普弗细胞不会随年龄变化，而老年大鼠内皮细胞中该酶的两种主要形式之一几乎消失。随着年龄增长，在长寿的实质细胞中观察到该酶形式非常明显的异质性模式。衰老实质细胞中多种形式酸性磷酸酶异质性的增加可能是翻译后修饰的结果。
