Wilson P D, Watson R, Knook D L
Gerontology. 1982;28(1):32-43. doi: 10.1159/000212509.
Hepatocytes, endothelial and Kupffer cells were isolated from young adult (3 month) and old (24 month) rat livers and the activities of some plasma membrane, endoplasmic reticulum, mitochondrial, lysosomal and soluble enzymes compared using biochemical and electron microscope cytochemical techniques. Age-associated changes included: a decrease in glucose-6-phosphatase activity both in hepatocytes and sinus lining cells; and increase in alkaline phosphatase in endothelial cells but a decrease in hepatocytes; reduced basal and glucagon-induced adenyl cyclase in hepatocytes and endothelial cells and an increase in the number of hepatocytes with gamma-glutamyl transferase reaction. Cytochemistry showed that heterogeneity may also be characteristic of senescence particularly with regard to hepatocyte glucose-6-phosphatase which was absent in some cells, low in many cells but high in some and gamma-glutamyl transferase which was normally lacking from hepatocytes but localised as large deposits of reaction product on the plasma membranes of occasional cells isolated from old donors.
从年轻成年（3个月）和老年（24个月）大鼠肝脏中分离出肝细胞、内皮细胞和库普弗细胞，并使用生化和电子显微镜细胞化学技术比较了一些质膜、内质网、线粒体、溶酶体和可溶性酶的活性。与年龄相关的变化包括：肝细胞和窦内衬细胞中葡萄糖-6-磷酸酶活性降低；内皮细胞中碱性磷酸酶增加，但肝细胞中减少；肝细胞和内皮细胞中基础和胰高血糖素诱导的腺苷酸环化酶减少，γ-谷氨酰转移酶反应阳性的肝细胞数量增加。细胞化学显示，异质性也可能是衰老的特征，特别是关于肝细胞葡萄糖-6-磷酸酶，在一些细胞中不存在，在许多细胞中较低，但在一些细胞中较高，以及γ-谷氨酰转移酶，正常情况下肝细胞中缺乏，但在从老年供体分离的偶尔细胞的质膜上定位为大量反应产物沉积物。
