Greb W, Strobel R
Toxicology. 1980;17(2):157-60. doi: 10.1016/0300-483x(80)90088-8.
Transformation of BHK 21 C 13 cells was evaluated by the ability of transformed cells to reproduce in semi solid agar (anchorage independent growth). The in vitro test system was carried out according to Styles with modifications. All compounds were tested with and without metabolic activation (S-9 mix). The results demonstrate the importance of a metabolizing system in this cell transformation assay.
通过转化细胞在半固体琼脂中繁殖的能力（不依赖贴壁生长）来评估BHK 21 C 13细胞的转化情况。体外测试系统按照Styles的方法进行，并做了修改。所有化合物均在有和没有代谢活化（S-9混合物）的情况下进行测试。结果证明了代谢系统在该细胞转化试验中的重要性。
