Amacher D E, Zelljadt I
Carcinogenesis. 1983;4(3):291-5. doi: 10.1093/carcin/4.3.291.
Nine chemicals classified as presumptive carcinogens on the basis of chronic rodent bioassays and one suspected human carcinogen which are reportedly not mutagenic to Salmonella typhimurium tester strains were tested for the ability to produce morphological transformation in Syrian hamster embryo cells in the absence of any exogenous source of metabolic activation. Acetamide, benzene, carbon tetrachloride, L-ethionine, monuron, piperonyl butoxide and trichloroethylene all induced positive morphological transformation in the presence of at least one of two medium and serum combinations as did the positive controls, ethyl methanesulfonate and benzo[a]pyrene. The remaining three chemicals, griseofulvin, isoniazid and trypan blue, did not induce morphological transformation under these same test conditions suggesting that they differ from the other seven chemicals in mechanism of action, target specificity or species susceptibility. Our results for seven of those ten selected chemicals in the clonal transformation assay using Syrian hamster embryo cells differ from their reported activity in the S. typhimurium point mutation assay. On the basis of this small sample, the Syrian hamster embryo transformation assay was a better predictor of the reported rodent bioassay results.
对九种根据慢性啮齿动物生物测定被归类为推定致癌物的化学物质以及一种据报道对鼠伤寒沙门氏菌测试菌株无致突变性的疑似人类致癌物，在不存在任何外源性代谢活化源的情况下，测试它们在叙利亚仓鼠胚胎细胞中产生形态转化的能力。乙酰胺、苯、四氯化碳、L-乙硫氨酸、灭草隆、胡椒基丁醚和三氯乙烯在两种培养基和血清组合中的至少一种存在下均诱导了阳性形态转化，阳性对照甲磺酸乙酯和苯并[a]芘也是如此。其余三种化学物质，即灰黄霉素、异烟肼和台盼蓝，在相同测试条件下未诱导形态转化，这表明它们在作用机制、靶标特异性或物种易感性方面与其他七种化学物质不同。我们在使用叙利亚仓鼠胚胎细胞的克隆转化试验中对这十种选定化学物质中的七种所得到的结果，与它们在鼠伤寒沙门氏菌点突变试验中报道的活性不同。基于这个小样本，叙利亚仓鼠胚胎转化试验对所报道的啮齿动物生物测定结果是一个更好的预测指标。
