Sanderson C J, Thomas J A
Immunology. 1978 Apr;34(4):771-80.
Antibody dependent cytotoxicity of chicken erythrocytes by purified rat eosinophils, neutrophils, macrophages and K cells has been compared by 51Cr release and time lapse microcinematography. Techniques have been developed for purifying these effector cell types. Both eosinophils and neutrophils cause rapid release of 51Cr from erythrocytes. Time lapse observations indicated that this was the result of phagocytosis. Eosinophils show rapid membrane movement and repeatedly engulf and regurgitate the erythrocytes. On the other hand, neutrophils become quiescent after phagocytosing erythrocytes, and remain quiescent until the remains of the cell are expelled. Neutrophils presumably have a mechanism for the release of soluble material, as 51Cr is released rapidly. Macrophages show a similar quiescence after phagocytosis, but in these cells there is apparently no rapid mechanism to expel material, as there is no significant 51Cr release over 20 h. K cells appear to damage chicken erythrocytes more slowly than they destroy tumour cells. Mast cells cause antibody-independent cytotoxicity which can be attributed to the release of toxic materials. None of these effector cells produced the type of lysis seen with antibody and complement.
通过51Cr释放法和延时显微电影摄影术，对纯化的大鼠嗜酸性粒细胞、中性粒细胞、巨噬细胞和K细胞对鸡红细胞的抗体依赖性细胞毒性进行了比较。已开发出纯化这些效应细胞类型的技术。嗜酸性粒细胞和中性粒细胞均可导致红细胞快速释放51Cr。延时观察表明，这是吞噬作用的结果。嗜酸性粒细胞表现出快速的膜运动，并反复吞噬和吐出红细胞。另一方面，中性粒细胞在吞噬红细胞后变得静止，并保持静止状态，直到细胞残骸被排出。中性粒细胞可能有一种释放可溶性物质的机制，因为51Cr释放迅速。巨噬细胞在吞噬后表现出类似的静止状态，但在这些细胞中，显然没有快速排出物质的机制，因为在20小时内没有明显的51Cr释放。K细胞对鸡红细胞的损伤似乎比对肿瘤细胞的破坏要慢。肥大细胞引起与抗体无关的细胞毒性，这可归因于有毒物质的释放。这些效应细胞均未产生抗体和补体所引起的那种细胞溶解类型。
