Lawson A D, Stevenson G T
Br J Cancer. 1983 Aug;48(2):227-37. doi: 10.1038/bjc.1983.178.
Guinea pig and mouse peritoneal macrophages formed antibody-dependent rosettes with guinea pig L2C leukaemic cells, but were unable either to phagocytose the cells or to kill them extracellularly as judged by the retention of 51Cr. Macrophages previously activated by BCG in vivo also failed to exhibit phagocytosis or cytoxicity towards the antibody-coated cells. These failures could not be attributed to deficient function of the macrophages nor to antigenic modulation of the L2C cells. The antibodies involved were capable of mediating lysis by complement, and ADCC by human leukocytes. However macrophages were cytostatic to antibody-coated L2C cells in that uptake of 3H-thymidine or 3H-deoxycytidine was abruptly and in some cases completely inhibited upon cell contact being established. Antigenic modulation which had proceeded sufficiently to protect against lysis by complement did not protect against cytostasis. Syngeneic macrophages had greater cytostatic activity than did allogeneic or xenogeneic. Macrophage activation by BCG did not result in significantly increased cytostasis. A univalent antibody derivative Fab/c was also capable of mediating cytostatis by the macrophages.
豚鼠和小鼠的腹腔巨噬细胞与豚鼠L2C白血病细胞形成抗体依赖性玫瑰花结，但无论是通过吞噬细胞还是通过51Cr的保留来判断细胞外杀伤，它们都无法做到。先前在体内被卡介苗激活的巨噬细胞对抗体包被的细胞也未表现出吞噬作用或细胞毒性。这些失败不能归因于巨噬细胞功能缺陷或L2C细胞的抗原调变。所涉及的抗体能够介导补体裂解以及人白细胞的抗体依赖性细胞介导的细胞毒性作用（ADCC）。然而，巨噬细胞对抗体包被的L2C细胞具有细胞生长抑制作用，因为一旦建立细胞接触，3H-胸腺嘧啶核苷或3H-脱氧胞苷的摄取就会突然且在某些情况下完全受到抑制。足以防止补体裂解的抗原调变并不能防止细胞生长抑制。同基因巨噬细胞比异基因或异种巨噬细胞具有更强的细胞生长抑制活性。卡介苗激活巨噬细胞并未导致细胞生长抑制作用显著增强。单价抗体衍生物Fab/c也能够介导巨噬细胞的细胞生长抑制作用。
