Richard C, Monteil H, Mégraud F, Chatelain R, Laurent B
Ann Biol Clin (Paris). 1981;39(1):9-15.
The authors compared the phenotype characteristics of 100 strains of Pseudomonas cepacia mostly of hospital origin to those of the typical mother strain (ATCC 25416). The morphological, cultural and biochemical characteristics (oxidase characteristics, ONPG, LDC, gelatinase and esculine were generally positive), nutritional characteristics (use of numerous organic compounds) and the antibiotypes (usually resistant to betalactamines, aminosides, polymyxins, tetracycline and furans) are reported, together with the differential diagnostic problems between P. cepacia and P. maltophilia (TTR+ methionine requirement). P. pseudomallei (LDC-, ODC-, ADH+, ONPG-) and Agrobacterium sp (urease+, LDC-, ODC-, absence of exoenzymes and use of citrate and malonate). An original classification in 8 biovars (characteristics used : esculine, nitratase, ONPG) permitted epidemiological marking of the strains and a study of their distribution.
作者将100株主要来源于医院的洋葱伯克霍尔德菌的表型特征与典型的母株（ATCC 25416）进行了比较。报告了其形态、培养和生化特征（氧化酶特征、邻硝基苯-β-D-半乳糖苷酶、赖氨酸脱羧酶、明胶酶和七叶苷通常呈阳性）、营养特征（利用多种有机化合物）以及抗菌谱型（通常对β-内酰胺类、氨基糖苷类、多粘菌素、四环素和呋喃类耐药），同时还提到了洋葱伯克霍尔德菌与嗜麦芽窄食单胞菌（TTR+，需要甲硫氨酸）之间的鉴别诊断问题。鼻疽伯克霍尔德菌（赖氨酸脱羧酶阴性、鸟氨酸脱羧酶阴性、精氨酸双水解酶阳性、邻硝基苯-β-D-半乳糖苷酶阴性）和土壤杆菌属（脲酶阳性、赖氨酸脱羧酶阴性、鸟氨酸脱羧酶阴性、无胞外酶，利用柠檬酸盐和丙二酸盐）。基于七叶苷、硝酸盐还原酶、邻硝基苯-β-D-半乳糖苷酶的8个生物变种的原始分类法，可对菌株进行流行病学标记并研究其分布情况。
