Kocan K M, Hsu K C, Hair J A, Ewing S A
Am J Vet Res. 1980 Dec;41(12):1977-81.
Cattle were inoculated with a Virginia isolate of Anaplasma marginale Theiler and served as an infective source for laboratory-reared Dermacentor variabilis (Say) nymphs. Transstadial transmission of A marginale was demonstrated by feeding the newly molted adult ticks on susceptible cattle and by inoculation of gut homogenates collected from adult ticks at postattachment day 6. A similar gut homogenate from the same group of ticks was caused to react with A marginale-bovine antisera that had been conjugated with ferritin. The homogenate contained organisms similar to those observed in the gut of adult feeding ticks that were infected as nymphs and the labeling of the outer membrane confirmed that organisms observed were A marginale. A gut homogenate prepared from control ticks did not cause infection when inoculated into a susceptible cow and no organisms were demonstrated.
用弗吉尼亚州的边缘无形体泰勒分离株接种牛，并将其作为实验室饲养的变异革蜱若虫的感染源。通过让新蜕皮的成年蜱吸食易感牛以及接种附着后第6天从成年蜱收集的肠道匀浆，证明了边缘无形体的经变态传播。使同一组蜱的类似肠道匀浆与已与铁蛋白偶联的边缘无形体-牛抗血清发生反应。该匀浆中含有与在若虫期感染的成年吸血蜱肠道中观察到的生物相似的生物，外膜的标记证实观察到的生物是边缘无形体。从对照蜱制备的肠道匀浆接种到易感母牛中不会引起感染，也未发现生物。
