Sunano S
Arch Int Pharmacodyn Ther. 1980 Nov;248(1):33-42.
The effects of temperature on high-K induced contracture of guinea-pig vas deferens were studied and compared with those of metabolic inhibitions. The rate of rise and fall of the phasic contraction decreased with the decrease in temperature. At 5 degrees C, the time to peak tension and half relaxation time increased to 25 times and to 38 times of those observed at 35 degrees C, respectively. The height of the phasic contraction increased by lowering of the temperature. The height of the tonic contraction also increased by lowering the temperature down to 15 degrees C but then decreased. Glucose removal and anoxia showed no effect on the phasic contraction, whereas the tonic contraction was abolished by these treatments. Metabolic inhibitors such as DNP, NaN3 or KCN, caused the depression of both the phasic and tonic contractions, being more effective on the tonic contraction. The tonic contraction was abolished before the phasic contraction was depressed by 50%. The time to peak tension and half relaxation time were not affected by metabolic inhibitions. These results suggest that low temperature causes the reduction in the rate of contractile reaction of muscle protein and in the rate of Ca accumulation and/or of Ca extrusion. Metabolic inhibition, on the other hand, caused reduction in the energy supply for the contraction and brought about depression of the contraction.
研究了温度对豚鼠输精管高钾诱导收缩的影响，并与代谢抑制的影响进行了比较。相收缩的上升和下降速率随温度降低而降低。在5℃时，达到峰值张力的时间和半松弛时间分别增加到在35℃时观察到的时间的25倍和38倍。相收缩的高度随温度降低而增加。强直收缩的高度在温度降至15℃时也增加，但随后降低。去除葡萄糖和缺氧对相收缩无影响，而这些处理可消除强直收缩。代谢抑制剂如二硝基苯酚（DNP）、叠氮化钠（NaN₃）或氰化钾（KCN）可导致相收缩和强直收缩均降低，对强直收缩的作用更明显。在相收缩降低50%之前，强直收缩就已被消除。达到峰值张力的时间和半松弛时间不受代谢抑制的影响。这些结果表明，低温导致肌肉蛋白收缩反应速率以及钙积累和/或钙外排速率降低。另一方面，代谢抑制导致收缩的能量供应减少，从而引起收缩降低。
