Hemagglutination test for adenoviruses using mouse erythrocytes.

Mouse erythrocytes were used, instead of rat erythrocytes, for typing adenoviruses by hemagglutination. Mouse erythrocytes were agglutinated by the prototype strains for adenovirus types 1, 2, 4, 5, 6, 8, 9 and 10 but not by types 3 and 7. Clear hemagglutination was observed without heterotypic antiserum by the microplate technique. No significant difference was observed in the hemagglutination titer using two strains of mice. Adenoviruses were identified by the hemagglutination inhibition test, using mouse erythrocytes and prototype strains 1, 2, 4, 5, 6 and 8. The use of mouse erythrocytes for typing adenoviruses is recommended.